饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
MTLRP; Growth Hormone-Releasing Peptide
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA991Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.9766-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.3255pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
- 價格 ¥ 1490 ¥ 1547 ¥ 1633 ¥ 1748 ¥ 1862 ¥ 2034 ¥ 2292 ¥ 2865 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-94 | 86 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 78-101 | 84 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-96% | 80-91% | 94-101% | 82-103% |
| EDTA plasma(n=5) | 89-103% | 78-88% | 79-101% | 79-102% |
| heparin plasma(n=5) | 94-103% | 78-94% | 95-104% | 87-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應用(僅供研究使用，不用于臨床診斷！) |
| UCEA991Ca | 饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA991Ca | 饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Archives of Oral Biology | Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents [PubMed: 21075359] |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533] |
| Journal of Physiology and Pharmacology | Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309] |
| Gene | The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192] |
| Hormone Research in Paediatrics | Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia [Pubmed: 23774845] |
| Surgical Innovation | Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model [Pubmed:25294792] |
| Obesity Surgery | Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model [Pubmed:25511751] |
| Obes Surg | Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420] |
| Surg Innov | Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model [PubMed: 25294792] |
| Nutrients.? | Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? [Pubmed:27240398] |
| World?Journal of Gastroenterology | Effects?of?sleeve?gastrectomy?with?jejuno-jejunal?or?jejuno-ileal?loop?onglycolipid?metabolism?in?diabetic?rats. [pubmed:27621579] |
| Ginekologia?Polska | Increased?serum?ghrelin?in?preeclampsia: is?ghrelin?a?friend?or a foe? [pubmed:27321099] |
| Gynecological Endocrinology | The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus [pubmed:27791436] |
| Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Role of serotonin hormone in weight regain after sleeve gastrectomy [pubmed:29228802] |
| Slovenian Veterinary?Research | EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH?… [Slovetres:Source] |
| The American Journal of the?Medical?Sciences | The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis [Pubmed:29753372] |
| American Journal of Perinatology | The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth [Pubmed: 30282106] |
| Stem Cells and Development | The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus [Pubmed: 30511904] |
| The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
| Arch Physiol Biochem | Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696] |
| Mol Med Rep | Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin?induced type 1 diabetes mouse?… [Pubmed: 32901885] |
| endocrine | Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate … [Pubmed: 32424681] |
| plos one | Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis [Pubmed: 30089154] |
| Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats [] | |
| International Journal of Livestock Research | Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers [] |
| iraqi journal of science | Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus [] |
| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women [] |
| Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study [34348809] |
| Nutrients | Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy [34959967] |
| scientific reports | Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats [34903800] |
| npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849] |
| Food Hydrocolloids | Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics [] |
