低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) ,etc. by FLIA (Flow Luminescence Immunoassay)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA798Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.01-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型Tissue homogenates, cell lysates and other biological fluids
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-99 | 96 |
| EDTA plasma(n=5) | 83-101 | 86 |
| heparin plasma(n=5) | 84-92 | 87 |
| sodium citrate plasma(n=5) | 93-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 90-101% | 91-105% | 80-89% |
| EDTA plasma(n=5) | 86-94% | 97-105% | 99-105% | 91-98% |
| heparin plasma(n=5) | 80-101% | 83-90% | 94-101% | 98-105% |
| sodium citrate plasma(n=5) | 95-103% | 99-105% | 79-99% | 83-92% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA798Rb01 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA798Rb51 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA798Rb21 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA798Rb | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA798Rb | 低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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