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血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay)

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

（注：單次混測多因子不超過8個(gè)指標(biāo) ）

編號ULMA380Hu
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實(shí)驗(yàn)方法競爭抑制
反應(yīng)時(shí)長1.5h
檢測范圍0.49-500pg/mL
靈敏度最小可檢測劑量小于等于0.163 pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	78-101	82
EDTA plasma(n=5)	90-101	94
heparin plasma(n=5)	98-105	102

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	85-101%	82-104%	82-103%	91-105%
EDTA plasma(n=5)	83-101%	78-102%	91-98%	92-101%
heparin plasma(n=5)	89-103%	80-103%	81-105%	88-102%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。

贈品

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實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Clinical Genetics	VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population [Wiley: source]
Human immunology	Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy [Pubmed: 23800435]
Headache: The Journal of Head and Face Pain	CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372]
World J Gastroenterol	Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide [NCBI: PMC4000510]
Cephalalgia?	Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine [Pubmed:24847167]
J Huazhong Univ Sci Technolog Med Sci.	Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients [Pubmed:25318892]
PLOS ONE	Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters [Pubmed:25647228]
PLOS ONE	Aqueous Fraction of [PubMed: 25647228]
Pharmacol Res	Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921]
World J Gastroenterol	Effects of sphincter of Oddi motility on the formation of cholesterol gallstones [Pubmed:27350732]
Annals of Allergy, Asthma and Immunology	Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748]
Cephalalgia.	Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233]
Headache	No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine [pubmed:27634731]
Sci Rep.	Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. [pubmed:28054652]
BMC Complementary and Alternative Medicine	[6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice [10.1186/s12906-017-1903-0]
Applied Biological Chemistry	Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3]
Journal of Periodontology	Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594]
Evidence-Based?Complementary and Alternative Medicine	Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946]
Revista do Instituto de Medicina Tropical de S?o Paulo	Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients [Pubmed: 30365640]
Journal of Veterinary Science	Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement [Pubmed: 30562932]
Science?of?Food?and?Agriculture	Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer [Pubmed: 30479041]
MOLECULAR BIOLOGY REPORTS	Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099]
Journal of Cystic Fibrosis	Disrupted local innervation results in less VIP expression in CF mice tissues [Pubmed: 32600901]
Biomedical Journal of Scientific & Technical Research	Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? []
Journal of tranditional chinese medical sciences	Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome []
J Cyst Fibros	VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD) [34034984]
Neurol Sci	Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study [34698942]
Endocr J	A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience [Pubmed:35644576]

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編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA380Hu01	血管活性腸肽(VIP)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
USPA380Hu01	血管活性腸肽(VIP)合成多肽	Immunogen; Blocking Peptide.
UPAA380Hu01	血管活性腸肽(VIP)多克隆抗體	WB; IHC; ICC; IP.
UMAA380Hu22	血管活性腸肽(VIP)單克隆抗體	WB
UMAA380Hu21	血管活性腸肽(VIP)單克隆抗體	WB
UCEA380Hu	血管活性腸肽(VIP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA380Hu	血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA380Hu11	血管活性腸肽(VIP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".