遮天,雪鹰领主

骨橋素(OPN)單克隆抗體

Monoclonal Antibody to Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

編號(hào)UMAA899Ra21
物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))-
Ig亞型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 -
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 326 760 ¥ 760 ¥ 1086 ¥ 2715 ¥ 10860
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)OPN的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別OPN。

用法

免疫印跡：0.2-2μg/mL;1:500-5000
免疫組織化學(xué)：5-20μg/mL;1:50-200
免疫細(xì)胞化學(xué)：5-20μg/mL;1:50-200
最佳稀釋倍數(shù)最終由用戶(hù)決定。

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Journal of Biomedical Materials Research Part A	Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture [PubMed: 19280635]
Osteoarthritis and Cartilage	Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients [PubMed: 19747583]
The Journal of Rheumatology	Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source]
International Journal of Pharmaceutics	Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742]
European Cells and matreials	The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source]
Journal of Equine Veterinary Science	Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source]
Basic & Clinical Pharmacology & Toxicology	Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source]
Journal of Cellular Biochemistry	Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552]
Naunyn-Schmiedeberg's Archives of Pharmacology	Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619]
J Endocrinol Invest.?	The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
FEBS Letters	Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352]
Gene	Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150]
Medicine (Baltimore)	An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Journal of Biomaterials and Tissue Engineering	Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel [Content: Asp]
Primasari	Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer [Index.Php: Amor]
Int J Biol Sci	Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817]
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao	Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost]
Am J Pathol.?	Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343]
circulation journal	Stenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
Journal of Cardiovascular Electrophysiology	Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817]
American Journal of Translational Research	Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. [pubmed:28337262]
Diabetes Care	Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824]
Biomedicine & pharmacotherapy	Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995]
Journal of?Alzheimers?Disease	Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720]
Indian?Journal?of?Nephrology	Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease [id:233806]
Carbon	In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells [Doi: 10.1016/j.carbon.2018.10.081]
current?eye?research	Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma. [Pubmed: 30994369]
Journal of Proteomics	Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma [Pubmed: 31629959]
Acta Biomaterialia	Inflammatory response to magnesium-based biodegradable implant materials [Pubmed: 31610341]
Journal of Clinical Medicine	Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and … [Pubmed: 32182704]
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES	Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats [Pubmed: 33010420]
[ENGINEERING OF BIOMATERIALS	Osteoconductive potential of PLGA/bioglass composite biomaterials []
Biomolecules	Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 [34070790]
	Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells []
Parasit Vectors	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?… [34857049]
Medicina (Kaunas)	Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980]

留言咨詢(xún)

編號(hào)	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA899Ra01	骨橋素(OPN)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UEPA899Ra61	骨橋素(OPN)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA899Ra61	骨橋素(OPN)活性蛋白	Cell?culture;?Activity?Assays.
UPAA899Ra01	骨橋素(OPN)多克隆抗體	IHC
UPAA899Ra06	骨橋素(OPN)多克隆抗體	WB; IHC
UMAA899Ra21	骨橋素(OPN)單克隆抗體	WB; IHC; ICC; IP.
USEA899Ra	骨橋素(OPN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA899Ra	骨橋素(OPN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA899Ra01	骨橋素(OPN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".